The present study is about Construct, Validation and Factor analysis of vocational interest scale and examine the course and job organization with this scale within male an female who work in public organization in Tehran city. For reach to this goal, jobs and vocational interests were classified and then primary form of this test was prepared. Then, an experimental sample completed it and then final form repaired and 450 males and females randomly selected who worked in the public organizations in Tehran completed this forms. Result of Exploratory factor analysis with principle component analysis,showed 19 .factors. It means Construct Validity. For Reliability used of Alpha Cronbach coefficient that was 0/9482. Between male and.female was For Criterion Validity used of jobs scale of Holand and Correlation between this scale and Holand's scale was 0/662. Between male and female was meaningful different in technical, artistic, accounting and suggestion subscales. Between different organizations was meaningful dijferenl in technical, music, accounting, suggestion and farming subscales. Just Social and literary Vocational interests had meaninglid relation with Social and literary courses. Just accounting, suggestion, employment jobs organization had meaningful relation with accounting, suggestion, employment interests. Just technical, social, literary, accounting, employment subscales had meaning relation with technical, social, literary, accounting, employment interests that expressed verbal.